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Nucleic Acids Res. 1990 Dec 11; 18(23): 6815–6819.
doi: 10.1093/nar/18.23.6815
PMCID: PMC332736
PMID: 2263446

Conversion of aminoacylation specificity from tRNA(Tyr) to tRNA(Ser) in vitro.

H Himeno, T Hasegawa, T Ueda, K Watanabe, and M Shimizu
Author information Copyright and License information PMC Disclaimer

Abstract

The discrimination mechanism between tRNA(Ser) and tRNA(Tyr) was studied using various in vitro transcripts of E. coli tRNATyr variants. The insertion of only two nucleotides into the variable stem of tRNA(Tyr) generates serine charging activity. The acceptor activities of some of the tRNA(Tyr) mutants with insertions in the long variable arm were enhanced by changes in nucleotides at positions 9 and/or 20B, which are possible elements for dictating the orientation of the long variable arm. These findings suggest that the long variable arm is involved in recognition by seryl-tRNA synthetase in spite of sequence and length variations shown within tRNA(Ser) isoacceptors, and eventually serves as a determinant for selection from other tRNA species. Changing the anticodon from GUA to the serine anticodon GGA resulted in a marked decrease in tyrosine charging activity, but this mutant did not show any serine charging activity. The discriminator base, the fourth base from the 3' end of tRNA, was also important for aminoacylation with tyrosine. Complete specificity change in vitro was facilitated by insertion of three nucleotides into the variable arm plus two nucleotide changes at positions 9 and 73.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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