Fig. 6

Effect of increased (2×Tg) Ube3a gene dosage on glutamate synapse number and postsynaptic glutamate receptor currents. Black, WT; red, Ube3a 2×Tg. (A) Synapse number, as measured by electron microscopy (P = 0.67, t test; n = 3 animals per group; 28 to 32 micrographs per animal). (B) Colocalized immunostaining of presynaptic (VGlut1) and postsynaptic (PSD-95) synaptic markers (scale bar, 1 μm; P = 0.8706, t test; n = 4 animals per group; eight or more micrographs per animal). (C) Spine number per dendrite length, as shown by Golgi staining (apical: P = 0.64; basal: P = 0.77, t test; n = 4 animals per group; ≥10 dendrites per animal). (D) Glutamate ionophoresis–induced AMPA and NMDA currents (AMPA: P = 0.46, t test; n = 5 to 6; NMDA: P = 0.97, t test; n = 3 to 6). (E) Fiber stimulation–evoked AMPA/NMDA traces (above, unscaled; below, scaled to WT) and ratio (P = 0.54, t test; n = 6 to 8).