Salt Stress Induced Variation in DNA Methylation Pattern and Its Influence on Gene Expression in Contrasting Rice Genotypes

Ratna Karan; Teresa DeLeon; Hanamareddy Biradar; Prasanta K. Subudhi

doi:10.1371/journal.pone.0040203

Figure 2

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Expression profiles of MSAP loci in rice under salt stress.

Salinity stressed shoot (A) and root (B) of six genotypes of rice, Bengal (Beng), IR29, Nipponbare (Nipp), Pokkali (Pokk), Nonabokra (Nona), and Geumgangbyeo (Geum) were used for quantifying the expression of MSAP loci relative to control. Real-time PCR analysis was performed using gene-specific primers. The expression of each gene in different RNA samples was normalized with the expression of internal control gene, rice elongation factor 1 α (eEF1 α). Fold changes in mRNA expression for each candidate gene in different genotypes were calculated relative to its control using ddCt method. The values represented are the mean of two biological replicates, each with three technical replicates. Error bars indicate the standard deviation.