Figure 4

Respiration rates of mitochondria isolated from the IR hearts treated with metformin (Met) in the presence or absence of GW6471 (GW). (A) State 3 at complex I measured in presence of 2.5 mM 2-oxoglutarate and 1 mM L-malate as substrates; (C) State 3 at complex II measured in presence of 2.5 mM succinate as a substrate; (E) Respiration rate at complex IV measured in presence of 10 mM ascorbate and 0.3 mM TMPD; (F) A direct effect of metformin (2 mM) on state 3 at complex I. Panels B and D show the respiratory control index (RCI) values calculated as the ratio of state 3 to state 2. * p < 0.05 IR+Met vs. IR; ⁺ p < 0.05 IR+GW or IR+Met+GW vs. IR+Met.