Skip to main content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Nucleic Acids Res. 1986 Dec 22; 14(24): 9653–9666.
doi: 10.1093/nar/14.24.9653
PMCID: PMC341326
PMID: 3468485

Transcriptional and post-transcriptional regulation of c-myc expression during the differentiation of murine erythroleukemia Friend cells.

N Mechti, M Piechaczyk, J M Blanchard, L Marty, A Bonnieu, P Jeanteur, and B Lebleu
Copyright and License information PMC Disclaimer

Abstract

c-myc RNA rapidly decreases to barely detectable levels in Friend erythroleukemia cells induced to differentiate upon the addition of dimethylsulfoxide. We show here that c-myc gene is down-regulated both at the transcriptional level presumably by a block in the elongation of primary transcripts and at the post-transcriptional level by an increase in the degradation of its mRNA.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.3M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

 
icon of scanned page 9653
9653
icon of scanned page 9654
9654
icon of scanned page 9655
9655
icon of scanned page 9656
9656
icon of scanned page 9657
9657
icon of scanned page 9658
9658
icon of scanned page 9659
9659
icon of scanned page 9660
9660
icon of scanned page 9661
9661
icon of scanned page 9662
9662
icon of scanned page 9663
9663
icon of scanned page 9664
9664
icon of scanned page 9665
9665
icon of scanned page 9666
9666
 

Images in this article

Image

Image
on p.9656

Image

Image
on p.9658

Image

Image
on p.9660

Image

Image
on p.9661

Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  • Land H, Parada LF, Weinberg RA. Cellular oncogenes and multistep carcinogenesis. Science. 1983 Nov 18;222(4625):771–778. [PubMed] [Google Scholar]
  • Marks PA, Rifkind RA. Erythroleukemic differentiation. Annu Rev Biochem. 1978;47:419–448. [PubMed] [Google Scholar]
  • Rifkind RA, Sheffery M, Profous-Juchelka HR, Reuben RC, Marks PA. Induction of globin gene expression during erythroid cell differentiation. Ann N Y Acad Sci. 1983;411:141–149. [PubMed] [Google Scholar]
  • Westin EH, Wong-Staal F, Gelmann EP, Dalla-Favera R, Papas TS, Lautenberger JA, Eva A, Reddy EP, Tronick SR, Aaronson SA, et al. Expression of cellular homologues of retroviral onc genes in human hematopoietic cells. Proc Natl Acad Sci U S A. 1982 Apr;79(8):2490–2494. [PMC free article] [PubMed] [Google Scholar]
  • Lachman HM, Skoultchi AI. Expression of c-myc changes during differentiation of mouse erythroleukaemia cells. Nature. 1984 Aug 16;310(5978):592–594. [PubMed] [Google Scholar]
  • Coppola JA, Cole MD. Constitutive c-myc oncogene expression blocks mouse erythroleukaemia cell differentiation but not commitment. Nature. 1986 Apr 24;320(6064):760–763. [PubMed] [Google Scholar]
  • Prochownik EV, Kukowska J. Deregulated expression of c-myc by murine erythroleukaemia cells prevents differentiation. Nature. 322(6082):848–850. [PubMed] [Google Scholar]
  • Dmitrovsky E, Kuehl WM, Hollis GF, Kirsch IR, Bender TP, Segal S. Expression of a transfected human c-myc oncogene inhibits differentiation of a mouse erythroleukaemia cell line. Nature. 1986 Aug 21;322(6081):748–750. [PubMed] [Google Scholar]
  • Watanabe T, Sariban E, Mitchell T, Kufe D. Human c-myc and N-ras expression during induction of HL-60 cellular differentiation. Biochem Biophys Res Commun. 1985 Feb 15;126(3):999–1005. [PubMed] [Google Scholar]
  • Dyson PJ, Littlewood TD, Forster A, Rabbitts TH. Chromatin structure of transcriptionally active and inactive human c-myc alleles. EMBO J. 1985 Nov;4(11):2885–2891. [PMC free article] [PubMed] [Google Scholar]
  • Einat M, Resnitzky D, Kimchi A. Close link between reduction of c-myc expression by interferon and, G0/G1 arrest. Nature. 1985 Feb 14;313(6003):597–600. [PubMed] [Google Scholar]
  • Klein G, Klein E. Evolution of tumours and the impact of molecular oncology. Nature. 1985 May 16;315(6016):190–195. [PubMed] [Google Scholar]
  • Stewart TA, Pattengale PK, Leder P. Spontaneous mammary adenocarcinomas in transgenic mice that carry and express MTV/myc fusion genes. Cell. 1984 Oct;38(3):627–637. [PubMed] [Google Scholar]
  • Adams JM, Harris AW, Pinkert CA, Corcoran LM, Alexander WS, Cory S, Palmiter RD, Brinster RL. The c-myc oncogene driven by immunoglobulin enhancers induces lymphoid malignancy in transgenic mice. Nature. 1985 Dec 12;318(6046):533–538. [PubMed] [Google Scholar]
  • Leder A, Pattengale PK, Kuo A, Stewart TA, Leder P. Consequences of widespread deregulation of the c-myc gene in transgenic mice: multiple neoplasms and normal development. Cell. 1986 May 23;45(4):485–495. [PubMed] [Google Scholar]
  • Keath EJ, Caimi PG, Cole MD. Fibroblast lines expressing activated c-myc oncogenes are tumorigenic in nude mice and syngeneic animals. Cell. 1984 Dec;39(2 Pt 1):339–348. [PubMed] [Google Scholar]
  • Kelly K, Cochran BH, Stiles CD, Leder P. Cell-specific regulation of the c-myc gene by lymphocyte mitogens and platelet-derived growth factor. Cell. 1983 Dec;35(3 Pt 2):603–610. [PubMed] [Google Scholar]
  • Campisi J, Gray HE, Pardee AB, Dean M, Sonenshein GE. Cell-cycle control of c-myc but not c-ras expression is lost following chemical transformation. Cell. 1984 Feb;36(2):241–247. [PubMed] [Google Scholar]
  • Armelin HA, Armelin MC, Kelly K, Stewart T, Leder P, Cochran BH, Stiles CD. Functional role for c-myc in mitogenic response to platelet-derived growth factor. Nature. 1984 Aug 23;310(5979):655–660. [PubMed] [Google Scholar]
  • Rapp UR, Cleveland JL, Brightman K, Scott A, Ihle JN. Abrogation of IL-3 and IL-2 dependence by recombinant murine retroviruses expressing v-myc oncogenes. Nature. 1985 Oct 3;317(6036):434–438. [PubMed] [Google Scholar]
  • Greenberg ME, Ziff EB. Stimulation of 3T3 cells induces transcription of the c-fos proto-oncogene. Nature. 1984 Oct 4;311(5985):433–438. [PubMed] [Google Scholar]
  • Endo T, Nadal-Ginard B. Transcriptional and posttranscriptional control of c-myc during myogenesis: its mRNA remains inducible in differentiated cells and does not suppress the differentiated phenotype. Mol Cell Biol. 1986 May;6(5):1412–1421. [PMC free article] [PubMed] [Google Scholar]
  • Bentley DL, Groudine M. A block to elongation is largely responsible for decreased transcription of c-myc in differentiated HL60 cells. Nature. 1986 Jun 12;321(6071):702–706. [PubMed] [Google Scholar]
  • Fahrlander PD, Piechaczyk M, Marcu KB. Chromatin structure of the murine c-myc locus: implications for the regulation of normal and chromosomally translocated genes. EMBO J. 1985 Dec 1;4(12):3195–3202. [PMC free article] [PubMed] [Google Scholar]
  • Dony C, Kessel M, Gruss P. Post-transcriptional control of myc and p53 expression during differentiation of the embryonal carcinoma cell line F9. Nature. 1985 Oct 17;317(6038):636–639. [PubMed] [Google Scholar]
  • Dean M, Levine RA, Campisi J. c-myc regulation during retinoic acid-induced differentiation of F9 cells is posttranscriptional and associated with growth arrest. Mol Cell Biol. 1986 Feb;6(2):518–524. [PMC free article] [PubMed] [Google Scholar]
  • Blanchard JM, Piechaczyk M, Dani C, Chambard JC, Franchi A, Pouyssegur J, Jeanteur P. c-myc gene is transcribed at high rate in G0-arrested fibroblasts and is post-transcriptionally regulated in response to growth factors. Nature. 1985 Oct 3;317(6036):443–445. [PubMed] [Google Scholar]
  • Knight E, Jr, Anton ED, Fahey D, Friedland BK, Jonak GJ. Interferon regulates c-myc gene expression in Daudi cells at the post-transcriptional level. Proc Natl Acad Sci U S A. 1985 Feb;82(4):1151–1154. [PMC free article] [PubMed] [Google Scholar]
  • Dani C, Mechti N, Piechaczyk M, Lebleu B, Jeanteur P, Blanchard JM. Increased rate of degradation of c-myc mRNA in interferon-treated Daudi cells. Proc Natl Acad Sci U S A. 1985 Aug;82(15):4896–4899. [PMC free article] [PubMed] [Google Scholar]
  • Dani C, Blanchard JM, Piechaczyk M, El Sabouty S, Marty L, Jeanteur P. Extreme instability of myc mRNA in normal and transformed human cells. Proc Natl Acad Sci U S A. 1984 Nov;81(22):7046–7050. [PMC free article] [PubMed] [Google Scholar]
  • Fort P, Marty L, Piechaczyk M, el Sabrouty S, Dani C, Jeanteur P, Blanchard JM. Various rat adult tissues express only one major mRNA species from the glyceraldehyde-3-phosphate-dehydrogenase multigenic family. Nucleic Acids Res. 1985 Mar 11;13(5):1431–1442. [PMC free article] [PubMed] [Google Scholar]
  • Rougeon F, Kourilsky P, Mach B. Insertion of a rabbit beta-globin gene sequence into an E. coli plasmid. Nucleic Acids Res. 1975 Dec;2(12):2365–2378. [PMC free article] [PubMed] [Google Scholar]
  • Stanton LW, Fahrlander PD, Tesser PM, Marcu KB. Nucleotide sequence comparison of normal and translocated murine c-myc genes. Nature. 1984 Aug 2;310(5976):423–425. [PubMed] [Google Scholar]
  • Hassouna N, Michot B, Bachellerie JP. The complete nucleotide sequence of mouse 28S rRNA gene. Implications for the process of size increase of the large subunit rRNA in higher eukaryotes. Nucleic Acids Res. 1984 Apr 25;12(8):3563–3583. [PMC free article] [PubMed] [Google Scholar]
  • Mechti N, Affabris E, Romeo G, Lebleu B, Rossi GB. Role of interferon and 2',5'-oligoadenylate synthetase in erythroid differentiation of Friend leukemia cells. Studies with interferon-sensitive and -resistant variants. J Biol Chem. 1984 Mar 10;259(5):3261–3265. [PubMed] [Google Scholar]
  • Piechaczyk M, Blanchard JM, Marty L, Dani C, Panabieres F, El Sabouty S, Fort P, Jeanteur P. Post-transcriptional regulation of glyceraldehyde-3-phosphate-dehydrogenase gene expression in rat tissues. Nucleic Acids Res. 1984 Sep 25;12(18):6951–6963. [PMC free article] [PubMed] [Google Scholar]
  • Piechaczyk M, Yang JQ, Blanchard JM, Jeanteur P, Marcu KB. Posttranscriptional mechanisms are responsible for accumulation of truncated c-myc RNAs in murine plasma cell tumors. Cell. 1985 Sep;42(2):589–597. [PubMed] [Google Scholar]
  • Schibler U, Hagenbüchle O, Wellauer PK, Pittet AC. Two promoters of different strengths control the transcription of the mouse alpha-amylase gene Amy-1a in the parotid gland and the liver. Cell. 1983 Jun;33(2):501–508. [PubMed] [Google Scholar]
  • Lindell TJ, Weinberg F, Morris PW, Roeder RG, Rutter WJ. Specific inhibition of nuclear RNA polymerase II by alpha-amanitin. Science. 1970 Oct 23;170(3956):447–449. [PubMed] [Google Scholar]
  • Alt F, Rosenberg N, Lewis S, Thomas E, Baltimore D. Organization and reorganization of immunoglobulin genes in A-MULV-transformed cells: rearrangement of heavy but not light chain genes. Cell. 1981 Dec;27(2 Pt 1):381–390. [PubMed] [Google Scholar]
  • Eick D, Piechaczyk M, Henglein B, Blanchard JM, Traub B, Kofler E, Wiest S, Lenoir GM, Bornkamm GW. Aberrant c-myc RNAs of Burkitt's lymphoma cells have longer half-lives. EMBO J. 1985 Dec 30;4(13B):3717–3725. [PMC free article] [PubMed] [Google Scholar]
  • Hay N, Skolnik-David H, Aloni Y. Attenuation in the control of SV40 gene expression. Cell. 1982 May;29(1):183–193. [PubMed] [Google Scholar]
  • Hay N, Aloni Y. Attenuation of late simian virus 40 mRNA synthesis is enhanced by the agnoprotein and is temporally regulated in isolated nuclear systems. Mol Cell Biol. 1985 Jun;5(6):1327–1334. [PMC free article] [PubMed] [Google Scholar]
  • Dalla-Favera R, Wong-Staal F, Gallo RC. Onc gene amplification in promyelocytic leukaemia cell line HL-60 and primary leukaemic cells of the same patient. Nature. 1982 Sep 2;299(5878):61–63. [PubMed] [Google Scholar]
  • Nevins JR, Blanchard JM, Darnell JE., Jr Transcription units of adenovirus type 2. Termination of transcription beyond the poly(A) addition site in early regions 2 and 4. J Mol Biol. 1980 Dec 15;144(3):377–386. [PubMed] [Google Scholar]
  • Fraser NW, Nevins JR, Ziff E, Darnell JE., Jr The major late adenovirus type-2 transcription unit: termination is downstream from the last poly(A) site. J Mol Biol. 1979 Apr 25;129(4):643–656. [PubMed] [Google Scholar]
  • Hofer E, Darnell JE., Jr The primary transcription unit of the mouse beta-major globin gene. Cell. 1981 Feb;23(2):585–593. [PubMed] [Google Scholar]
  • Hagenbüchle O, Wellauer PK, Cribbs DL, Schibler U. Termination of transcription in the mouse alpha-amylase gene Amy-2a occurs at multiple sites downstream of the polyadenylation site. Cell. 1984 Oct;38(3):737–744. [PubMed] [Google Scholar]
  • Krowczynska A, Yenofsky R, Brawerman G. Regulation of messenger RNA stability in mouse erythroleukemia cells. J Mol Biol. 1985 Jan 20;181(2):231–239. [PubMed] [Google Scholar]
  • Aviv H, Voloch Z, Bastos R, Levy S. Biosynthesis and stability of globin mRNA in cultured erythroleukemic Friend cells. Cell. 1976 Aug;8(4):495–503. [PubMed] [Google Scholar]
  • Bastos RN, Volloch Z, Aviv H. Messenger RNA population analysis during erythroid differentiation: a kinetical approach. J Mol Biol. 1977 Feb 25;110(2):191–203. [PubMed] [Google Scholar]
  • Harris LJ, Remmers EF, Brodeur P, Riblet R, D'Eustachio P, Marcu KB. c-myc Gene rearrangements involving gamma immunoglobulin heavy chain gene switch regions in murine plasmacytomas. Nucleic Acids Res. 1983 Dec 10;11(23):8303–8315. [PMC free article] [PubMed] [Google Scholar]
Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press
-