Uncoupling the roles of synaptotagmin I as a dual Ca2+ sensor during endo- and exocytosis of synaptic vesicles

Jun Yao; Sung E. Kwon; Jon D. Gaffaney; F. Mark Dunning; Edwin R. Chapman

doi:10.1038/nn.3013

Figure 5

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C2A and C2B are redundant Ca²⁺ sensors for endocytosis. (a) Diagram showing the Ca²⁺ ligand mutant forms of pHluorin tagged syt1 employed for fluorescence imaging experiments. (b) Normalized fluorescence changes in syt1 KO neurons expressing sypHy (n = 52), pH-syt1^FL (n = 64) or Ca²⁺ ligand mutants of pH-syt1^FL (pH-syt1^4DN, n = 48; C2A^2DN-C2B, n = 72; C2A-C2B^2DN, n = 61; C2B^2DN-C2A, n = 50; C2B-C2A^2DN, n = 60). ‘n’ is total number of boutons pooled from 4 coverslips. (c) Bar graph summarizing the τ values for endocytosis in (B). ** p<0.001. Error bars represent SEM.