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Proc Natl Acad Sci U S A. 1980 Dec; 77(12): 7024–7028.
PMCID: PMC350433
PMID: 6938951
Specific in vitro transcription of conalbumin gene is drastically decreased by single-point mutation in T-A-T-A box homology sequence.
Abstract
A single-point mutation, consisting of a T-to-G transversion, was made in the third nucleotide of the conalbumin gene T-A-T-A-A-A-A homology sequence (the T-A-T-A or Goldberg-Hogness box). In an in vitro system, specific transcription of the mutant DNA was drastically decreased compared to the normal gene. This down-mutation is consistent with the idea that the T-A-T-A box is an important element for specific initiation of transcription.
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Selected References
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- Weil PA, Luse DS, Segall J, Roeder RG. Selective and accurate initiation of transcription at the Ad2 major late promotor in a soluble system dependent on purified RNA polymerase II and DNA. Cell. 1979 Oct;18(2):469–484. [PubMed] [Google Scholar]
- Wu GJ. Adenovirus DNA-directed transcription of 5.5S RNA in vitro. Proc Natl Acad Sci U S A. 1978 May;75(5):2175–2179. [PMC free article] [PubMed] [Google Scholar]
- Wasylyk B, Kédinger C, Corden J, Brison O, Chambon P. Specific in vitro initiation of transcription on conalbumin and ovalbumin genes and comparison with adenovirus-2 early and late genes. Nature. 1980 Jun 5;285(5764):367–373. [PubMed] [Google Scholar]
- Cochet M, Gannon F, Hen R, Maroteaux L, Perrin F, Chambon P. Organization and sequence studies of the 17-piece chicken conalbumin gene. Nature. 1979 Dec 6;282(5739):567–574. [PubMed] [Google Scholar]
- Proudfoot NJ. Eukaryotic promoters? Nature. 1979 May 31;279(5712):376–376. [PubMed] [Google Scholar]
- Gannon F, O'Hare K, Perrin F, LePennec JP, Benoist C, Cochet M, Breathnach R, Royal A, Garapin A, Cami B, et al. Organisation and sequences at the 5' end of a cloned complete ovalbumin gene. Nature. 1979 Mar 29;278(5703):428–434. [PubMed] [Google Scholar]
- Flavell RA. The transcription of eukaryotic genes. Nature. 1980 Jun 5;285(5764):356–357. [PubMed] [Google Scholar]
- Maxam AM, Gilbert W. Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980;65(1):499–560. [PubMed] [Google Scholar]
- Jay E, Bambara R, Padmanabhan R, Wu R. DNA sequence analysis: a general, simple and rapid method for sequencing large oligodeoxyribonucleotide fragments by mapping. Nucleic Acids Res. 1974 Mar;1(3):331–353. [PMC free article] [PubMed] [Google Scholar]
- Herrmann R, Neugebauer K, Pirkl E, Zentgraf H, Schaller H. Conversion of bacteriophage fd into an efficient single-stranded DNA vector system. Mol Gen Genet. 1980 Jan;177(2):231–242. [PubMed] [Google Scholar]
- Hutchison CA, 3rd, Phillips S, Edgell MH, Gillam S, Jahnke P, Smith M. Mutagenesis at a specific position in a DNA sequence. J Biol Chem. 1978 Sep 25;253(18):6551–6560. [PubMed] [Google Scholar]
- Gillam S, Jahnke P, Astell C, Phillips S, Hutchison CA, 3rd, Smith M. Defined transversion mutations at a specific position in DNA using synthetic oligodeoxyribonucleotides as mutagens. Nucleic Acids Res. 1979 Jul 11;6(9):2973–2985. [PMC free article] [PubMed] [Google Scholar]
- Gillam S, Smith M. Site-specific mutagenesis using synthetic oligodeoxyribonucleotide primers: I. Optimum conditions and minimum ologodeoxyribonucleotide length. Gene. 1979 Dec;8(1):81–97. [PubMed] [Google Scholar]
- Gillam S, Smith M. Site-specific mutagenesis using synthetic oligodeoxyribonucleotide primers: II. In vitro selection of mutant DNA. Gene. 1979 Dec;8(1):99–106. [PubMed] [Google Scholar]
- Razin A, Hirose T, Itakura K, Riggs AD. Efficient correction of a mutation by use of chemically synthesized DNA. Proc Natl Acad Sci U S A. 1978 Sep;75(9):4268–4270. [PMC free article] [PubMed] [Google Scholar]
- Rosenberg M, Court D. Regulatory sequences involved in the promotion and termination of RNA transcription. Annu Rev Genet. 1979;13:319–353. [PubMed] [Google Scholar]
- Siebenlist U, Simpson RB, Gilbert W. E. coli RNA polymerase interacts homologously with two different promoters. Cell. 1980 Jun;20(2):269–281. [PubMed] [Google Scholar]
- Pribnow D. Nucleotide sequence of an RNA polymerase binding site at an early T7 promoter. Proc Natl Acad Sci U S A. 1975 Mar;72(3):784–788. [PMC free article] [PubMed] [Google Scholar]
- Schaller H, Gray C, Herrmann K. Nucleotide sequence of an RNA polymerase binding site from the DNA of bacteriophage fd. Proc Natl Acad Sci U S A. 1975 Feb;72(2):737–741. [PMC free article] [PubMed] [Google Scholar]
- Grosschedl R, Birnstiel ML. Identification of regulatory sequences in the prelude sequences of an H2A histone gene by the study of specific deletion mutants in vivo. Proc Natl Acad Sci U S A. 1980 Mar;77(3):1432–1436. [PMC free article] [PubMed] [Google Scholar]
- Benoist C, Chambon P. Deletions covering the putative promoter region of early mRNAs of simian virus 40 do not abolish T-antigen expression. Proc Natl Acad Sci U S A. 1980 Jul;77(7):3865–3869. [PMC free article] [PubMed] [Google Scholar]
- Baker CC, Herisse J, Courtois G, Galibert F, Ziff E. Messenger RNA for the Ad2 DNA binding protein: DNA sequences encoding the first leader and heterogenity at the mRNA 5' end. Cell. 1979 Oct;18(2):569–580. [PubMed] [Google Scholar]
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