Ablation of Calcineurin Aβ Reveals Hyperlipidemia and Signaling Cross-talks with Phosphodiesterases

Hee Yun Suk; Chen Zhou; Teddy T. C. Yang; Hong Zhu; Raymond Y. L. Yu; Opeyemi Olabisi; XiaoYong Yang; Deborah Brancho; Ja-Young Kim; Philipp E. Scherer; Philippe G. Frank; Michael P. Lisanti; John W. Calvert; David J. Lefer; Jeffery D. Molkentin; Alessandra Ghigo; Emilio Hirsch; Jianping Jin; Chi-Wing Chow

doi:10.1074/jbc.M112.419150

FIGURE 3.

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Lipoprotein clearance, triglyceride synthesis, and lipid mobilization in CnAβ^−/− and CnAβ^+/+ mice. A, triglyceride accumulation in CnAβ^−/− and CnAβ^+/+ mice (n = 6) was determined upon injection of Triton WR1339 (500 mg/kg), which inhibits lipid clearance. The levels of triglyceride were determined at the times indicated and presented. B, mRNA in liver of 8-week-old CnAβ^−/− and CnAβ^+/+ mice (n = 6) were amplified by quantitative RT-PCR to determine the expression of key SREBP target genes involved in de novo triglyceride/cholesterol synthesis. The number of cycles required to reach threshold was represented as relative mRNA expression. Fold difference of 1 is equivalent to 2-fold change. *, p < 0.05; #, p < 0.005. C and D, the levels of triglyceride (C) and free fatty acids (FFA; D) of CnAβ^−/− and CnAβ^+/+ mice (n = 6) upon fasting were determined at the times indicated.