FIGURE 6.

Potentiation of PKA activation in CnAβ^−/− mice or upon CsA treatment is sensitive to general phosphodiesterase inhibitor. MEFs (n = 3) isolated from CnAβ^−/− (A and B) and CnAβ^+/+ (A–D) mice were challenged with β-adrenergic receptor agonist Iso (10 μm), in the absence (A and C) or presence (B and D) of the phosphodiesterase inhibitor IBMX (0.5 mm). C–F, MEFs isolated from CnAβ^+/+ mice (n = 3) (C and D) or differentiated 3T3/L1 adipocytes (E and F) were pretreated (CsA) or not (Control) with calcineurin inhibitor CsA (5 μm) for 1 h before stimulation with adrenergic receptor agonists, in the absence (−) or presence (+) of IBMX. The cell extracts prepared were used to determine activation of PKA by immunoblotting analysis using phospho-PKA motif antibodies (phospho-PKA substrates). Expression of tubulin is shown as a control.