Figure 1

(A) Comparative intersection analysis of elevated transcripts in murine hepatocytes expressing YAP and HuH-7 cells after YAP silencing (transfection of si#1) revealed 15 inversely regulated candidate genes.⁵ Selected candidates were validated in HuH-7 by real-time PCR using an additional YAP-specific siRNA (si#2). (B) Western blot analysis of YAP, Jag-1, and NICD, 24 h after siRNA-mediated knockdown of YAP in HuH-7 cells. (C) Relative mRNA levels of the Notch pathway target gene Hes-1 in HuH-7 cells 24 h after transfection of YAP-specific siRNAs. Untreated cells (cont.) were used for calibration. (D) Western blot analysis of PLC/PRF/5 protein extracts after vector-based overexpression of YAP^wt. Amounts of Jag-1, YAP^wt, and Hes-1 were detected 24 h after transfection. Untreated (cont.) and empty vector (mock)-transfected cells served as controls. For (B) and (D) actin was used as loading control.