Fig. 1

(A) The conditioned medium from arsenic-transformed cells stimulates tube formation by HUVECs and (B) Stably expressing miR-200b in arsenic-transformed cells impairs tube formation by HUVECs. Representative images of tube formation by HUVECs induced by the conditioned media from indicated cells. The conditioned media were prepared for tube formation assay as described in Materials and Methods. The quantifications of formed tube branches was carried out as described in Materials and Methods and presented as total number of tube branches per well (means ± standard deviations, n=3). Scale bar=200 μm. * p<0.05, compared to p53^lowHBECs (A) and to As-p53^lowHBEC-GFP (B). Similar results were obtained in two additional experiments. (C) Enhanced angiogenesis is detected in mouse xenograft tumors produced by inoculation of arsenic-transformed cells (As-p53^lowHBEC-GFP).Representative overlaid fluorescent images from anti-CD31 immunofluorescence staining (red color) and nucleus DAPI staining (blue color) in mouse xenograft tissues resulting from injection of As-p53^lowHBEC-GFP or As-p53^lowHBEC-GFP-200b cells. Tissue section preparation and anti-CD31 staining were carried out as described in Materials and Methods. The CD31 staining was quantified and presented as the number of CD31 positive-stained vessel structures per field of view (FOV) as described in Materials and Methods (means ± standard deviations, n=3). Scale bar=100 μm. * p<0.05, compared to the As-p53^lowHBEC-GFP group.