Fig. 3
(A) Representative images of arsenic-transformed vector control cells (As-p53lowHBEC-GFP) shown in a bright field (upper panel) and their β-catenin immunofluorescence staining (lower pane). White arrows point to representative nucleus stainings of β-catenin. (B) Representative images of arsenic-transformed miR-200b stably expressing cells (As-p53lowHBEC-GFP-200b) shown in a bright field (upper panel) and their β-catenin immunofluorescence staining (lower pane). White arrows point to representative cytoplasmic membrane stainings of β-catenin. β-Catenin immunofluorescence staining was carried out as described in Materials and Methods. Scale bar=50 μm. (C) Representative Western blot analysis of cellular β-catenin, c-myc, cyclin D1 and VEGF protein levels. Western blot was carried out as described in Materials and Methods. (D) Q-PCR analysis of cellular c-myc, cyclin D1 and VEGF mRNA levels (means ± standard deviations, n=3). Q-PCR analysis was performed as described in Materials and Methods. * p<0.05, compared to the As-p53lowHBEC-GFP cells. Similar results were obtained in two additional experiments.