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Alignment of E. coli DnaX, YcaJ, and RuvB (clustal x multiple sequence alignment program, v. 1.8, http://www.ebi.ac.uk/clustalw/). The sequences of YcaJ, RuvB, and DnaX all contain well-conserved nucleotide-binding sites with Walker A (GxxxxGKT/S) and Walker B (Dexx) motifs. The Zn-binding motif of DnaX is absent in YcaJ, but the putative ATPase sensor motifs (29) are present. Colors represent types of amino acids.

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