PMC full text:
Published online 2013 Aug 13. doi: 10.1128/mBio.00524-13
TABLE 2
Observed frequency of nsp-related intracellular features compared to the expected frequency
| Transfected | Phenotype observeda | Expected transfection efficiency (%)b | Approximate diam (µm) | Expected frequency (%)c | Observed frequency (%) |
|---|---|---|---|---|---|
| nsp3 | DMB/MGV | 70 | 4 | 19 | 11 |
| nsp3C | DMB/MGV | 70 | 4 | 19 | 6 |
| nsp6 | MTOCV | 70 | 4 | 19 | 17 |
| nsp3 + nsp4 | DMB/MGV | 21 | 4 | 6 | 5 |
| MLB | 49 | 2 | 7 | 11 | |
| nsp3 + nsp6 | DMB/MGV | 70 | 4 | 19 | 4 |
| MTOCV | 70 | 4 | 19 | 18 | |
| nsp4 + nsp6 | MTOCV | 21 | 4 | 6 | 3 |
| nsp3 + nsp4 + nsp6 | DMB/MGV | 21 | 4 | 6 | 1 |
| MTOCV | 21 | 4 | 6 | 5 | |
| MLB | 15 | 2 | 2 | 12 | |
| DMV cluster | 34 | 0.5 | 1 | 1 | |
| nsp3C + nsp4 + nsp6 | DMB/MGV | 70 | 4 | 9 | 13 |
| MTOCV | 54 | 4 | 7 | 6 |
aBoth nsp3-induced membrane phenotypes are combined under the heading DMB/MGV.
bData are based on an assumed independent 70% transfection efficiency for each plasmid, combining probabilities for plasmid combinations expected to result in the given phenotype; e.g., in nsp3-nsp6 transfection, the nsp6 phenotype is expected in nsp6 single transfectants (21% of cells) plus nsp3-nsp6 double transfectants (49% of cells) because nsp3 and nsp6 phenotypes appear to be independent, whereas nsp4-nsp6 transfection would be expected to result only in the nsp6 phenotype in nsp6 single transfectants (21% of cells) because nsp4 appeared to counteract the nsp6 phenotype.
cCalculated as expected transfection efficiency × (average diameter of feature/15-µm average cell diameter).