PGC-1 Coactivators Regulate MITF and the Tanning Response

Jonathan Shoag; Rizwan Haq; Mingfeng Zhang; Laura Liu; Glenn C. Rowe; Aihua Jiang; Nicole Koulisis; Caitlin Farrel; Christopher I. Amos; Qingyi Wei; Jeffrey E. Lee; Jiangwen Zhang; Thomas S. Kupper; Abrar A. Qureshi; Rutao Cui; Jiali Han; David E. Fisher; Zoltan Arany

doi:10.1016/j.molcel.2012.10.027

Figure 2

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PGC-1 Coactivators Induce MITF-M Expression

(A–D) mRNA expression of the indicated genes in B16-F10 cells stably overexpressing PGC-1α versus vector control (A) and lentiviral knockdown of PGC-1α in B16-F10 (B), UACC-257 (C), and normal human melanocytes (D).

(E) Relative activity of luciferase expressed from the MITF-M promoter, cotransfected into UACC-257 cells with plasmids expressing the indicated proteins.

(F) Relative luciferase activity, as in (E).

(G) Occupancy by PGC-1α on the MITF-M promoter at the indicated sites. Black bars correspond to binding near the SOX and CRE sites. IgG, immunoglobulin G.

(H) Relative MITF-M mRNA in B16-F10 cells stably overexpressing PGC-1α or PGC-1β. CTRL, control.

(I) mRNA of indicated genes in UACC-257 cells following lentiviral knockdown of PGC-1β.

(J) MITF-M promoter activity in UACC-257 cells expressing the indicated proteins.

*p < 0.05. Error bars represent mean ± SD.