Figure 2

Inhibition of PPP2R5C expression in K562R cells by RNA interference. Detection of the transfection efficiencies in K562R cells by fluorescence microscopy (Bar = 50 μm) and flow cytometry (positive cells are shown in the P2 domain). A and B: Alexa Red Oligo-transfected K562R cells (fluorescence microscope); C: Alexa Red Oligo-transfected K562R cells (FCM); D and E: Mock-transfected K562R cells (fluorescence microscope); F: Mock-transfected K562R cells (FCM). G: Suppression of PPP2R5C mRNA expression as measured by qRT–PCR after nucleofection with PPP2R5C siRNAs (3 μg). *, p < 0.05 compared with expression in cells treated with control non-silencing RNA. H: PPP2R5C protein level in K562R cells 72 h after nucleofection with PPP2R5C siRNAs (3 μg). Non-treated cells (nc), mock-transfected (mock), and scrambled non-silencing control RNA (SC)-treated cells were used as controls.