Figure 3

Biological consequences of PPP2R5C silencing. (A) Absorbance of PPP2R5C-siRNA799- and PPP2R5C-siRNA991-treated and control cells (K562) at different time points as measured by the CCK-8 method. The results represent mean values of three independent experiments. *, p < 0.001 compared with the scrambled non-silencing control RNA-treated cells. (B) Induction of apoptosis by PPP2R5C suppression in K562 cells 72 h after nucleofection with PPP2R5C-siRNA (3 μg). **, p = 0.004 compared with non-silencing control RNA-treated cells. (C) Hoechst 33258-stained K562 nuclei 72 h after transfection in the PPP2R5C siRNA groups were mostly densely stained, demonstrating a white color, while the normal nuclei in the control groups were light blue under a fluorescence microscope. Bar = 50 μm.