Fig. 3.

Inhibition of NKT cell function by PS-1145. (A and B) Splenocyte cultures were activated for 3 days with α-GalCer (200 ng/ml) in the presence of various amounts of the IKK2-inhibitor PS-1145. After 3 days, proliferation was monitored by [³H]thymidine incorporation (A), and IFN-γ levels were determined by ELISA (B). (C and D) Mice were pretreated with PS-1145 or carrier perorally 1.5 h before injection of 2 μgof α-GalCer. Three hours after injection of α-GalCer, serum was removed to determine IL-4 and IFN-γ levels by ELISA. Each bar represents the mean calculated from five mice, error bars indicate the SEM. *, P ≤ 0.05; Student's t test.