Differential dependence of CD4+CD25+ regulatory and natural killer-like T cells on signals leading to NF-κB activation

Marc Schmidt-Supprian; Jane Tian; Ethan P. Grant; Manolis Pasparakis; René Maehr; Huib Ovaa; Hidde L. Ploegh; Anthony J. Coyle; Klaus Rajewsky

doi:10.1073/pnas.0400885101

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Proc Natl Acad Sci U S A. 2004 Mar 30; 101(13): 4566–4571.

Published online 2004 Mar 16. doi: 10.1073/pnas.0400885101

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Table 1.

Absolute numbers of T cell populations in Bcl10^-/-, Pkcθ^-/-, and control mice

Cell type	Organ	Bcl10^-/-	WT mixed	Pkcθ^-/-	WT BL/6
CD4⁺CD25⁺	Thy (× 10⁶)	0.02 ± 0.03	0.21 ± 0.09	0.04 ± 0.02	0.43 ± 0.26
CD4⁺CD25⁺CD45Rb^int	Spl (× 10⁶)	0.05 ± 0.03	1.35 ± 0.29	0.32 ± 0.07	1.4 ± 0.53
CD4⁺CD44⁺	Spl (× 10⁶)	0.57 ± 0.29	2.31 ± 0.66	0.98 ± 0.22	1.91 ± 0.99
CD4⁺CD44⁺CD62L^low	Spl (× 10⁶)	0.52 ± 0.31	2.6 ± 0.57	—	—
CD4⁺CD25^-CD45Rb^low	Spl (× 10⁶)	0.2 ± 0.06	0.77 ± 0.25	0.21 ± 0.06	0.87 ± 0.37
NKT	Thy (× 10⁶)	0.11 ± 0.06	0.34 ± 0.15	0.11 ± 0.04	0.46 ± 0.07
NKT	Spl (× 10⁶)	0.06 ± 0.03	0.85 ± 0.05	0.44 ± 0.13	0.47 ± 0.1
NKT	Liver (× 10³)	0.21 ± 0.4	4.5 ± 0.15	2.7 ± 1.59	2.7 ± 0.58
NKT	BM (× 10³)	0.18 ± 0.12	2.26 ± 0.14	3.6 ± 1.36	3.6 ± 1.23
NK	Spl (× 10⁶)	0.69 ± 0.36	2.03 ± 0.17	—	—
NK	Liver (× 10³)	0.53 ± 0.13	2.95 ± 1.07	—	—

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Cell types were distinguished by FACS according to the indicated cell surface markers. NKT cells comprise CD 1d/α-GalCer tetramer-positive T cells, and NK cells are NK1.1⁺TCRβ^- cells. Staining with tetramers identifies a subset, although the major one, of Nk1.1⁺ T cells. This fact, together with the lower background of staining with αTCRβ/tetramer compared with αTCRβ/αNK1.1, explains the lower absolute NKT cell numbers detected in control mice shown in this table, compared with NKT cell numbers in control mice shown in Fig. 2B. Data are shown as mean ± SD calculated from 4-5 mice.