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Proc Natl Acad Sci U S A. 1986 Aug; 83(16): 6159–6163.
doi: 10.1073/pnas.83.16.6159
PMCID: PMC386459
PMID: 2426711

Detection of antibodies to human T-lymphotropic virus type III by using a synthetic peptide of 21 amino acid residues corresponding to a highly antigenic segment of gp41 envelope protein.

J J Wang, S Steel, R Wisniewolski, and C Y Wang
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Abstract

A peptide of 21 amino acids with the sequence Arg-Ile-Leu-Ala-Val-Glu-Arg-Tyr-Leu-Lys-Asp-Gln-Gln-Leu-Leu-Gly-Ile-Trp- Gly-Cys - Ser encoded by a segment in the env gene of human T-lymphotropic virus type III (HTLV-III), corresponding to amino acids 586-606 of the precursor envelope glycoprotein, has been synthesized by the Merrifield solid-phase method. Combined serological and chemical analyses of this peptide and related peptides have revealed the importance of certain amino acid residues in the antigenic determinant of the relevant peptide. Enzyme immunoassay (EIA) employing this peptide as an antigen adsorbent was shown to reproducibly detect antibodies in sera of patients with HTLV-III infection. This assay provided positive results with all sera that were reactive with gp41 envelope protein of HTLV-III in electrophoretic immunoblot analysis. Thus far, no false-positive sera have been encountered in control populations. Our EIA with this peptide as the coating antigen is shown to have advantages over that with the whole HTLV-III virus as an immunoadsorbent.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  • Popovic M, Sarngadharan MG, Read E, Gallo RC. Detection, isolation, and continuous production of cytopathic retroviruses (HTLV-III) from patients with AIDS and pre-AIDS. Science. 1984 May 4;224(4648):497–500. [PubMed] [Google Scholar]
  • Gallo RC, Salahuddin SZ, Popovic M, Shearer GM, Kaplan M, Haynes BF, Palker TJ, Redfield R, Oleske J, Safai B, et al. Frequent detection and isolation of cytopathic retroviruses (HTLV-III) from patients with AIDS and at risk for AIDS. Science. 1984 May 4;224(4648):500–503. [PubMed] [Google Scholar]
  • Barré-Sinoussi F, Chermann JC, Rey F, Nugeyre MT, Chamaret S, Gruest J, Dauguet C, Axler-Blin C, Vézinet-Brun F, Rouzioux C, et al. Isolation of a T-lymphotropic retrovirus from a patient at risk for acquired immune deficiency syndrome (AIDS). Science. 1983 May 20;220(4599):868–871. [PubMed] [Google Scholar]
  • Levy JA, Hoffman AD, Kramer SM, Landis JA, Shimabukuro JM, Oshiro LS. Isolation of lymphocytopathic retroviruses from San Francisco patients with AIDS. Science. 1984 Aug 24;225(4664):840–842. [PubMed] [Google Scholar]
  • Muesing MA, Smith DH, Cabradilla CD, Benton CV, Lasky LA, Capon DJ. Nucleic acid structure and expression of the human AIDS/lymphadenopathy retrovirus. Nature. 1985 Feb 7;313(6002):450–458. [PubMed] [Google Scholar]
  • Ratner L, Haseltine W, Patarca R, Livak KJ, Starcich B, Josephs SF, Doran ER, Rafalski JA, Whitehorn EA, Baumeister K, et al. Complete nucleotide sequence of the AIDS virus, HTLV-III. Nature. 1985 Jan 24;313(6000):277–284. [PubMed] [Google Scholar]
  • Wain-Hobson S, Sonigo P, Danos O, Cole S, Alizon M. Nucleotide sequence of the AIDS virus, LAV. Cell. 1985 Jan;40(1):9–17. [PubMed] [Google Scholar]
  • Sanchez-Pescador R, Power MD, Barr PJ, Steimer KS, Stempien MM, Brown-Shimer SL, Gee WW, Renard A, Randolph A, Levy JA, et al. Nucleotide sequence and expression of an AIDS-associated retrovirus (ARV-2). Science. 1985 Feb 1;227(4686):484–492. [PubMed] [Google Scholar]
  • Sarngadharan MG, Popovic M, Bruch L, Schüpbach J, Gallo RC. Antibodies reactive with human T-lymphotropic retroviruses (HTLV-III) in the serum of patients with AIDS. Science. 1984 May 4;224(4648):506–508. [PubMed] [Google Scholar]
  • Cheingsong-Popov R, Weiss RA, Dalgleish A, Tedder RS, Shanson DC, Jeffries DJ, Ferns RB, Briggs EM, Weller IV, Mitton S, et al. Prevalence of antibody to human T-lymphotropic virus type III in AIDS and AIDS-risk patients in Britain. Lancet. 1984 Sep 1;2(8401):477–480. [PubMed] [Google Scholar]
  • Kitchen LW, Barin F, Sullivan JL, McLane MF, Brettler DB, Levine PH, Essex M. Aetiology of AIDS--antibodies to human T-cell leukaemia virus (type III) in haemophiliacs. Nature. 1984 Nov 22;312(5992):367–369. [PubMed] [Google Scholar]
  • Safai B, Sarngadharan MG, Groopman JE, Arnett K, Popovic M, Sliski A, Schüpbach J, Gallo RC. Seroepidemiological studies of human T-lymphotropic retrovirus type III in acquired immunodeficiency syndrome. Lancet. 1984 Jun 30;1(8392):1438–1440. [PubMed] [Google Scholar]
  • Kalyanaraman VS, Cabradilla CD, Getchell JP, Narayanan R, Braff EH, Chermann JC, Barré-Sinoussi F, Montagnier L, Spira TJ, Kaplan J, et al. Antibodies to the core protein of lymphadenopathy-associated virus (LAV) in patients with AIDS. Science. 1984 Jul 20;225(4659):321–323. [PubMed] [Google Scholar]
  • Gazzard BG, Shanson DC, Farthing C, Lawrence AG, Tedder RS, Cheingsong-Popov R, Dalgleish A, Weiss RA. Clinical findings and serological evidence of HTLV-III infection in homosexual contacts of patients with AIDS and persistent generalised lymphadenopathy in London. Lancet. 1984 Sep 1;2(8401):480–483. [PubMed] [Google Scholar]
  • Brun-Vezinet F, Rouzioux C, Barre-Sinoussi F, Klatzmann D, Saimot AG, Rozenbaum W, Christol D, Gluckmann JC, Montagnier L, Chermann JC. Detection of IgG antibodies to lymphadenopathy-associated virus in patients with AIDS or lymphadenopathy syndrome. Lancet. 1984 Jun 9;1(8389):1253–1256. [PubMed] [Google Scholar]
  • Laurence J, Brun-Vezinet F, Schutzer SE, Rouzioux C, Klatzmann D, Barré-Sinoussi F, Chermann JC, Montagnier L. Lymphadenopathy-associated viral antibody in AIDS. Immune correlations and definition of a carrier state. N Engl J Med. 1984 Nov 15;311(20):1269–1273. [PubMed] [Google Scholar]
  • Spira TJ, Des Jarlais DC, Marmor M, Yancovitz S, Friedman S, Garber J, Cohen H, Cabradilla C, Kalyanaraman VC. Prevalence of antibody to lymphadenopathy-associated virus among drug-detoxification patients in New York. N Engl J Med. 1984 Aug 16;311(7):467–468. [PubMed] [Google Scholar]
  • Tsoukas C, Gervais F, Shuster J, Gold P, O'Shaughnessy M, Robert-Guroff M. Association of HTLV-III antibodies and cellular immune status of hemophiliacs. N Engl J Med. 1984 Dec 6;311(23):1514–1515. [PubMed] [Google Scholar]
  • Chang NT, Chanda PK, Barone AD, McKinney S, Rhodes DP, Tam SH, Shearman CW, Huang J, Chang TW, Gallo RC, et al. Expression in Escherichia coli of open reading frame gene segments of HTLV-III. Science. 1985 Apr 5;228(4695):93–96. [PubMed] [Google Scholar]
  • Chang NT, Huang J, Ghrayeb J, McKinney S, Chanda PK, Chang TW, Putney S, Sarngadharan MG, Wong-Staal F, Gallo RC. An HTLV-III peptide produced by recombinant DNA is immunoreactive with sera from patients with AIDS. Nature. 1985 May 9;315(6015):151–154. [PubMed] [Google Scholar]
  • Crowl R, Ganguly K, Gordon M, Conroy R, Schaber M, Kramer R, Shaw G, Wong-Staal F, Reddy EP. HTLV-III env gene products synthesized in E. coli are recognized by antibodies present in the sera of AIDS patients. Cell. 1985 Jul;41(3):979–986. [PubMed] [Google Scholar]
  • Allan JS, Coligan JE, Barin F, McLane MF, Sodroski JG, Rosen CA, Haseltine WA, Lee TH, Essex M. Major glycoprotein antigens that induce antibodies in AIDS patients are encoded by HTLV-III. Science. 1985 May 31;228(4703):1091–1094. [PubMed] [Google Scholar]
  • Barin F, McLane MF, Allan JS, Lee TH, Groopman JE, Essex M. Virus envelope protein of HTLV-III represents major target antigen for antibodies in AIDS patients. Science. 1985 May 31;228(4703):1094–1096. [PubMed] [Google Scholar]
  • Laursen RA. Solid-phase Edman degradation. An automatic peptide sequencer. Eur J Biochem. 1971 May 11;20(1):89–102. [PubMed] [Google Scholar]
  • Tam JP, Heath WF, Merrifield RB. SN 1 and SN 2 mechanisms for the deprotection of synthetic peptides by hydrogen fluoride. Studies to minimize the tyrosine alkylation side reaction. Int J Pept Protein Res. 1983 Jan;21(1):57–65. [PubMed] [Google Scholar]
  • Hopp TP, Woods KR. Prediction of protein antigenic determinants from amino acid sequences. Proc Natl Acad Sci U S A. 1981 Jun;78(6):3824–3828. [PMC free article] [PubMed] [Google Scholar]
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