Single-cell and population level viral infection dynamics revealed by phageFISH, a method to visualize intracellular and free viruses

Elke Allers; Cristina Moraru; Melissa B Duhaime; Erica Beneze; Natalie Solonenko; Jimena Barrero-Canosa; Rudolf Amann; Matthew B Sullivan

doi:10.1111/1462-2920.12100

PMC full text:

Environ Microbiol. 2013 Aug; 15(8): 2306–2318.

Published online 2013 Mar 14. doi: 10.1111/1462-2920.12100

Copyright/LicenseRequest permission to reuse

Figure 3

An external file that holds a picture, illustration, etc.
Object name is emi0015-2306-f3.jpg

Open in a separate window

Epifluorescence micrograph of free virus after phageFISH (red, Alexa₅₉₄) and SYBR staining (green).On the left: probe targeting a viral gene, on the right: negative control gene probe.Top: overlay of SYBR Green and phageFISH, centre: phageFISH only, bottom: SYBR Green only. Scale bar indicates 2 μm.

Images in this article

Click on the image to see a larger version.