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Proc Natl Acad Sci U S A. 1971 Mar; 68(3): 557–560.
doi: 10.1073/pnas.68.3.557
PMCID: PMC388987
PMID: 5276761

Biochemical Studies on Adenovirus Multiplication, XIX. Resolution of Late Viral RNA Species in the Nucleus and Cytoplasm

J. Thomas Parsons,* Jacqueline Gardner, and Maurice Green†
Author information Copyright and License information PMC Disclaimer

Abstract

Late after infection of cultured human cells (KB) with adenovirus type 2, the nucleus contains heterogeneous viral RNA species ranging in size from 10 to 43 S. Four viral RNA species found in the nucleus (36, 38, 40, and 43 S) are synthesized predominantly during a 15-min labeling period with [3H]uridine, while smaller RNA species accumulate when labeling is continued for longer periods. In contrast, 6-8 viral RNA species, of sedimentation coefficient from 10 to 29 S, are found in the cytoplasm after a 30-min pulse label and a 2-hr chase. DNA-RNA hybridization-competition experiments demonstrate that viral RNA sequences present in nuclear 36-43S RNA are also present in cytoplasmic and polyribosomal RNA, suggesting that at least some of the cytoplasmic viral-specific RNA molecules are derived by cleavage of high molecular weight precursors from the nucleus.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  • Yuan R, Meselson M. A specific complex between a restriction endonuclease and its DNA substrate. Proc Natl Acad Sci U S A. 1970 Feb;65(2):357–362. [PMC free article] [PubMed] [Google Scholar]
  • Thomas DC, Green M. Biochemical studies on adenovirus multiplication. XV. Transcription of the adenovirus type II genome during productive infection. Virology. 1969 Oct;39(2):205–210. [PubMed] [Google Scholar]
  • Fujinaga K, Mak S, Green M. A method for determining the fraction of the viral genome transcribed during infection and its application to adenovirus-infected cells. Proc Natl Acad Sci U S A. 1968 Jul;60(3):959–966. [PMC free article] [PubMed] [Google Scholar]
  • Maizel JV, Jr, White DO, Scharff MD. The polypeptides of adenovirus. II. Soluble proteins, cores, top components and the structure of the virion. Virology. 1968 Sep;36(1):126–136. [PubMed] [Google Scholar]
  • White DO, Scharff MD, Maizel JV., Jr The polypeptides of adenovirus. 3. Synthesis in infected cells. Virology. 1969 Jul;38(3):395–406. [PubMed] [Google Scholar]
  • Horwitz MS, Maizel JV, Jr, Scharff MD. Molecular weight of Adenovirus type 2 hexon polypeptide. J Virol. 1970 Oct;6(4):569–571. [PMC free article] [PubMed] [Google Scholar]
  • Fujinaga K, Green M. Mechanism of viral carcinogenesis by DNA mammalian viruses. V. Properties of purified viral-specific RNA from human adenovirus-induced tumor cells. J Mol Biol. 1968 Jan 14;31(1):63–73. [PubMed] [Google Scholar]
  • DATTA P, GEST H, SEGAL HL. EFFECTS OF FEEDBACK MODIFIERS ON THE STATE OF AGGREGATION OF HOMOSERINE DEHYDROGENASE OF RHODOSPIRILLUM RUBRUM. Proc Natl Acad Sci U S A. 1964 Jan;51:125–130. [PMC free article] [PubMed] [Google Scholar]
  • Bader JP, Steck TL. Analysis of the ribonucleic acid of murine leukemia virus. J Virol. 1969 Oct;4(4):454–459. [PMC free article] [PubMed] [Google Scholar]
  • Kakefuda T, Bader JP. Electron microscopic observations on the ribonucleic acid of murine leukemia virus. J Virol. 1969 Oct;4(4):460–474. [PMC free article] [PubMed] [Google Scholar]
  • Katz E, Moss B. Formation of a vaccinia virus structural polypeptide from a higher molecular weight precursor: inhibition by rifampicin. Proc Natl Acad Sci U S A. 1970 Jul;66(3):677–684. [PMC free article] [PubMed] [Google Scholar]
  • Wagner EK, Roizman B. RNA synthesis in cells infected with herpes simplex virus. II. Evidence that a class of viral mRNA is derived from a high molecular weight precursor synthesized in the nucleus. Proc Natl Acad Sci U S A. 1969 Oct;64(2):626–633. [PMC free article] [PubMed] [Google Scholar]
  • Martin MA, Byrne JC. Sedimentation properties of simian virus 40-specific ribonucleic acid present in green monkey cells during productive infection and in mouse cells undergoing abortive infection. J Virol. 1970 Oct;6(4):463–469. [PMC free article] [PubMed] [Google Scholar]
  • SCHERRER K, LATHAM H, DARNELL JE. Demonstration of an unstable RNA and of a precursor to ribosomal RNA in HeLa cells. Proc Natl Acad Sci U S A. 1963 Feb 15;49:240–248. [PMC free article] [PubMed] [Google Scholar]
  • Weinberg RA, Loening U, Willems M, Penman S. Acrylamide gel electrophoresis of HeLa cell nucleolar RNA. Proc Natl Acad Sci U S A. 1967 Sep;58(3):1088–1095. [PMC free article] [PubMed] [Google Scholar]
  • Birnstiel M, Speirs J, Purdom I, Jones K, Loening UE. Properties and composition of the isolated ribosomal DNA satellite of Xenopus laevis. Nature. 1968 Aug 3;219(5153):454–463. [PubMed] [Google Scholar]
  • Weinberg RA, Penman S. Processing of 45 s nucleolar RNA. J Mol Biol. 1970 Jan 28;47(2):169–178. [PubMed] [Google Scholar]
  • Scherrer K, Marcaud L, Zajdela F, London IM, Gros F. Patterns of RNA metabolism in a differentiated cell: a rapidly labeled, unstable 60S RNA with messenger properties in duck erythroblasts. Proc Natl Acad Sci U S A. 1966 Nov;56(5):1571–1578. [PMC free article] [PubMed] [Google Scholar]
  • Attardi B, Attardi G. A membrane-associated RNA of cytoplasmic origin in HeLa cells. Proc Natl Acad Sci U S A. 1967 Sep;58(3):1051–1058. [PMC free article] [PubMed] [Google Scholar]
  • Warner JR, Soeiro R, Birnboim HC, Girard M, Darnell JE. Rapidly labeled HeLa cell nuclear RNA. I. Identification by zone sedimentation of a heterogeneous fraction separate from ribosomal precursor RNA. J Mol Biol. 1966 Aug;19(2):349–361. [PubMed] [Google Scholar]
  • Penman S, Smith I, Holtzman E. Ribosomal RNA synthesis and processing in a particulate site in the HeLa cell nucleus. Science. 1966 Nov 11;154(3750):786–789. [PubMed] [Google Scholar]
  • Stévenin J, Mandel P, Jacob M. Relationship between nuclear giant-size dRNA and microsomal dRNA of rat brain. Proc Natl Acad Sci U S A. 1969 Feb;62(2):490–497. [PMC free article] [PubMed] [Google Scholar]
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