FIGURE 1.

Microarray analysis (Affymetrix Drosophila Genome 2.0) of gene expression changes in Drosophila S2 cells after depletion of Cdk8, CycC, Med12, or Med13. A, hierarchical clustering according to Euclidean distance of indicated samples using 135 probe sets with ≥3-fold change between any 2 of 22 microarray samples. Colors indicate expression change (log 2) as compared with average of all samples. ctrl, control. B, Pearson's correlation matrix of indicated samples based on average log 2-fold change of 2119 probe sets that show ≥1.5-fold changes between any of the 22 microarray samples. Correlation is shown by colors as indicated (0, no correlation; 1, total positive correlation). C, heat map of genes with at least 3-fold change in expression after depletion of Cdk8, CycC, Med12, or Med13 as indicated. Colors indicate expression change (log 2) to average of control. D, overlap of genes with ≥1.5-fold (p < 0.02) increased (red) or decreased (blue) mRNA levels after depletion of both Cdk8 and CycC (Cdk8-CycC) or both Med12 and Med13 (Med12-Med13). E, Western blotting analysis with antibodies against Cdk8, CycC, Med12, and Med13 72 h following treatment with the indicated dsRNAs. Unspecific background bands serving as loading controls are indicated by asterisks; Cdk8 and CycC detection was from the same blot.