FIGURE 4.

Med12 and Med13 are required for GATA-dependent transcription. A, luciferase activity of CG14629-luc normalized to transfection control pRLnull-copiaLTR in control and Srp-depleted S2 cells as indicated. Error bars represent S.D. in two representative experiments with three biological replicates each. B, luciferase activity of p4xPO45-luc (black) and CG14629-luc (gray) reporters normalized to transfection control pRLnull-copiaLTR in Lozenge-overexpressing (Lz-v5) and control cells. Error bars represent S.D. in two experiments. C, schematic of the CG14629-luc and CG14629-ΔGATA-luc reporters showing distribution and orientation of GATA sites, where sites with point mutations are in gray. Activity of the reporters and pGL3-basic normalized to transfection control pRLnull-copiaLTR is indicated in the columns below as average and S.D. of five (pGL3-basic) or nine independent experiments. D, columns indicate relative decrease of CG14629-luc (black) or CG14629-ΔGATA-luc (gray) activity in Srp-depleted S2 cells as compared with GFP dsRNA control. Error bars represent S.D. of six experiments. E, luciferase activity of CG14629-luc normalized to transfection control pRLnull-copiaLTR following dsRNA treatment as indicated. Error bars represent S.D. in two representative experiments with three biological replicates each. F, columns indicate relative decrease of CG14629-luc (black) or CG14629-ΔGATA-luc (gray) activity in S2 cells treated with the indicated dsRNA as compared with GFP dsRNA control. Error bars represent S.D. of at least eight experiments. **, p < 0.01, ***, p < 0.001.