Microenvironmental Stiffness Enhances Glioma Cell Proliferation by Stimulating Epidermal Growth Factor Receptor Signaling

Vaibhavi Umesh; Andrew D. Rape; Theresa A. Ulrich; Sanjay Kumar

doi:10.1371/journal.pone.0101771

Figure 1

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Microenvironmental stiffness regulates glioma cell proliferation.

Effect of ECM rigidity on proliferation of U373-MG (A) and U87-MG (B) cells. Results represent quantification of n>10,000 cells for at least three substrates per condition by flow cytometry, where the percentage of dividing cells was determined as the average percentage of cells staining positive for BrdU incorporation. *, P<0.05 with respect to 119 kPa.