Figure 6
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Reduced ZPA expression of Shh in Nipbl
+/− mouse limb buds.
(A–B) Whole mount ISH for Shh in the hindlimb buds of E10.5 wild type (A) and Nipbl +/− (B) mice. In these dorsal views, anterior to the top, the left and right ZPA are seen as localized patches of staining on the posteriolateral edge of each bud (arrowheads). The ZPA of the right forelimb bud is also visible in the background (arrows). Scale bar = 0.5 mm. (C) Quantification of ISH patterns from 5 wild type and 5 mutant embryos. Limb bud and ZPA size were estimated from image areas. Hybridization intensity was measured as mean pixel intensity in the ZPA multiplied by ZPA area. Data are normalized to wild type values. * = p<0.05, ** = p<0.01.