Loss of histone H4K20 trimethylation predicts poor prognosis in breast cancer and is associated with invasive activity

Yuhki Yokoyama; Ayaka Matsumoto; Miki Hieda; Yoshimi Shinchi; Eri Ogihara; Mai Hamada; Yu Nishioka; Hiroshi Kimura; Katsuhide Yoshidome; Masahiko Tsujimoto; Nariaki Matsuura

doi:10.1186/bcr3681

Figure 4

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Knockdown of SUV420H2-activated cell invasion in normal mammary epithelial cells, MCF10A. (A) SUV420H2 and GAPDH mRNA levels in MCF10A were analyzed by PCR and agarose gel (left) and real-time PCR (right). (B) Invasive activities of SUV420H2-knockdown cells. Results are presented as means ± standard deviation (SD). (C) siRNA against SUV420H2 was transfected into MCF10A, and the expression levels of SUV420H2 and GAPDH mRNA were examined by PCR and agarose gel (left) and real-time PCR (right).