Characterization of Cre Recombinase Activity for In Vivo Targeting of Adipocyte Precursor Cells

Katherine C. Krueger; Maria José Costa; Hongqing Du; Brian J. Feldman

doi:10.1016/j.stemcr.2014.10.009

Figure 2

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Fabp4-Cre Recombinase Activity in Adipose Stromal Populations, Culture, and Tissue Sections

(A) Flow cytometry profile of Fabp4-Cre;Rosa-EYFP male mice (n = 3), where each n is one animal. Data are represented as mean ± SEM.

(B) Flow cytometry profile of Fabp4-Cre:Rosa-EYFP female mice (n = 3), where each n is one animal. Data are represented as mean ± SEM.

(C) Expression of EYFP in cultured cells, prior to or after adipogenic induction. The estimated percentage of EYFP⁺ cells or adipocytes is indicated. Scale bar represents 0.2 mm.

(D) Expression of EYFP and perilipin (red), a marker of adipocytes, in sections of adipose tissue. Arrows indicate nonrecombined adipocytes. Arrowheads indicate putative endothelial structures labeled by EYFP. Scale bar represents 0.1 mm.

(E) Expression of EYFP and CD31 (red) in sections of adipose tissue or skeletal muscle. Scale bar represents 0.1 mm.

Mac, macrophage. See also Figure S2.