Figure 1

Synthetic poly(rA) (lane 1) and poly(dT) (lane 2) oligos were annealed, and the samples were run on a 1% agarose gel. Arrows indicate the annealed poly(rA):poly(dT) (lane 3) RNA:DNA hybrid.

Different amounts of synthetic annealed poly(rA):poly(dT) hybrid was spotted on a nitrocellulose membrane and probed with anti-hybrid S9.6 antibody.

Poly(rA):poly(dT) was either undigested (1) or digested with DNase I (2), RNase A (3) or RNase H (4), and the digested samples were analyzed on a 1% agarose gel.

Human peripheral blood mononuclear cells (PBMCs) were transfected with or without poly(rA), poly(dT) or poly(rA):poly(dT) hybrid or poly(dA):poly(dT) dsDNA for 6 h. RT–PCR was performed to check for the expression of IFNB. Figure shows values from two biological replicates. Data plot shows mean with SEM.

Human PBMCs from two different donors were transfected with or without poly(rA), poly(dT) or poly(rA):poly(dT) for 16 h, and the amount of secreted IP-10 was measured by ELISA. Each experiment was performed in duplicate. Data plot shows mean with SEM.

Differentiated human monocyte THP-1 cells were transfected with or without poly(rA):poly(dT) hybrid, poly(dA):poly(dT) dsDNA and RT–PCR was performed to check for the expression of IFNB. Each experiment was performed in duplicate. Data are representative of one of two independent experiments. Data plot shows mean with SEM.

Differentiated human monocyte THP-1 cells with Gaussia luciferase knockin under IFIT1 promoter were transfected with or without poly(rA):poly(dT) hybrid, poly(dA):poly(dT) dsDNA and luciferase assay was performed. Data shown are from two biological replicates. Data plot shows mean with SEM.

Differentiated human monocyte THP-1 cells with Gaussia luciferase knockin under IFIT1 promoter were transfected with different amounts of poly(rA):poly(dT) hybrid or poly(dA):poly(dT) dsDNA and luciferase assay was performed. Data shown are from two biological replicates. Data plot shows mean with SEM.