The beneficial effects of AMP kinase activation against oxidative stress are associated with prevention of PPARα-cyclophilin D interaction in cardiomyocytes

Giselle Barreto-Torres; Jessica Soto Hernandez; Sehwan Jang; Adlín R. Rodríguez-Muñoz; Carlos A. Torres-Ramos; Alexei G. Basnakian; Sabzali Javadov

doi:10.1152/ajpheart.00414.2014

Fig. 5.

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Effects of Met on H₂O₂-induced cyclophilin D (CypD)-peroxisome proliferator-activated receptor-α (PPARα) interaction. Cell lysates from each group were immunoprecipitated (IP) with anti-CypD (A) or anti-PPARα (B) antibodies. The complexes were subjected to SDS-PAGE followed by immunoblotting (IB) with CypD, PPARα, adenine nucleotide translocator (ANT), and voltage-dependent anion channel (VDAC). Representative immunoblots (A and B, top) show the effects of oxidative stress on the interaction between PPARα and CypD in the presence and absence of Met. Quantitative results (A and B, bottom) were expressed as percent change compared with control. *P < 0.05 vs. C; +P < 0.05 vs. H₂O₂; n = 3 to 4 per group.