Fig. 8.
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Effects of oxidative stress on mtDNA abundance and damage in H9c2 cardiomyocytes in the presence or absence of Met. Cells were treated with varying concentrations of H2O2 for 1 h followed by DNA isolation and quantitative PCR analysis. A: mtDNA abundance. B: mtDNA damage. C: effect of Met on H2O2-induced mtDNA damage. Graphs represent the average of two PCR reactions performed in duplicate. AU, arbitrary units. *P < 0.05 and **P < 0.01 vs. C; +P < 0.05 vs. H2O2.