Fig. 8.

Effects of oxidative stress on mtDNA abundance and damage in H9c2 cardiomyocytes in the presence or absence of Met. Cells were treated with varying concentrations of H₂O₂ for 1 h followed by DNA isolation and quantitative PCR analysis. A: mtDNA abundance. B: mtDNA damage. C: effect of Met on H₂O₂-induced mtDNA damage. Graphs represent the average of two PCR reactions performed in duplicate. AU, arbitrary units. *P < 0.05 and **P < 0.01 vs. C; +P < 0.05 vs. H₂O₂.