Figure 5

FcRL4+ B cells represent a pro-inflammatory population with a unique cytokine profile. (A) Gating strategy for FcRL4+ and FcRL4− B cell fractions sorted from RA synovial fluid using a MoFlo FACS sorter. Populations were sorted to a minimum purity of 95%. (B) Real-time PCR analysis of cytokine mRNA in FcRL4+ and FcRL4− B cells shows that FcRL4+ B cell express higher levels of RANKL and TNF-α than the FcRL4− B cell population. Conversely, FcRL4− B cells express higher levels of LT-β, B cell activating factor (BAFF), transforming growth factor-β (TGF-β), VEGF-α, CCL3, CCL4 and CCL5. *p<0.05, **p<0.01, Wilcoxon matched paired test; n=9.