Response to Nodal morphogen gradient is determined by the kinetics of target gene induction

Julien Dubrulle; Benjamin M Jordan; Laila Akhmetova; Jeffrey A Farrell; Seok-Hyung Kim; Lilianna Solnica-Krezel; Alexander F Schier

doi:10.7554/eLife.05042

PMC full text:

eLife. 2015; 4: e05042.

Published online 2015 Apr 14. doi: 10.7554/eLife.05042

Copyright/LicenseRequest permission to reuse

Table 1.

β-actin::GFP-Smad2 transgene rescues smad2/smad2 adult lethality

DOI: http://dx.doi.org/10.7554/eLife.05042.004

smad2/+ X smad2/+; Tg(GFP-Smad2)/+
Genotype	+/+	Tg(gfp-smad2)/+
+/+	11 (37%)	4 (15%)
smad2/+	19 (63%)	17 (60%)
smad2/smad2	0 (0%)	7 (25%)

Open in a separate window

smad2/+ fish were crossed to smad2/+; Tg(GFP-Smad2)/+ fish and their progeny was raised to adulthood and genotyped for smad2 and for Tg(GFP-Smad2). The only recovered adult progeny homozygous for smad2 contains a copy of the GFP-Smad2 transgene.