PMC full text:
Published online 2015 Jan 28. doi: 10.1074/jbc.M114.596288
TABLE 1
The two intrinsic activities of DnaK
The two intrinsic activities of DnaK are as follows: ATPase and peptide substrate binding activities.
| ATPase activity kcat | Peptide binding Kd | |
|---|---|---|
| ×10−2/min | μm | |
| WT | 1.04 ± 0.076 | 1.37 ± 0.04 |
| R56A | 3.61 ± 0.260 | 1.25 ± 0.06 |
| T301A | 0.89 ± 0.089 | 1.28 ± 0.06 |
| N537A | 0.91 ± 0.094 | 0.72 ± 0.07 |
| D540A | 0.99 ± 0.109 | 0.75 ± 0.06 |