Figure 5

Effect of environmental temperature and light–dark phase on body temperature and energy homeostasis. A. Body composition. B. Energy expenditure as a function of environmental temperature during the light phase. Regression lines were calculated using the 18–28 °C Ta range. C. Energy expenditure as a function of environmental temperature during the light phase. Data as in B, but normalized with BMR set to 1. D. Effect of environmental temperature on the conductance, calculated as EE/(Tb − Ta) during light phase. E. Dependence of the heat conductance on lean and fat weight. Conductance = 0.0304 + (0.000884 * Fat) − (0.000339 * Lean); SE are 0.0114, 0.000191, and 0.000501, respectively, adjusted R² = 0.71. F. Dependence of BMR on lean and fat weight. BMR = −0.00626 + (0.00113 * fat) + (0.00694 * lean); SE are 0.0682, 0.00114, and 0.00299, respectively, adjusted R² = 0.58. In B-D, chow-fed data are circles and solid lines; HFD data are squares and dashed lines. Data are mean ± SEM of 6 chow-fed and 5 high-fat-fed male C57BL/6J mice (*, p < 0.05; ***, p < 0.001).