Deletion of Kinin B2 Receptor Alters Muscle Metabolism and Exercise Performance

Felipe C. G. Reis; Anderson S. Haro; Aline V. N. Bacurau; Sandro M. Hirabara; Frederick Wasinski; Milene S. Ormanji; José B. N. Moreira; Beatriz H. Kiyomoto; Clelia R. A. Bertoncini; Patricia C. Brum; Rui Curi; Michael Bader; Reury F. P. Bacurau; João B. Pesquero; Ronaldo C. Araújo

doi:10.1371/journal.pone.0134844

Fig 3

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Indirect calorimetry and mitochondrial activity.

(A) Oxygen consumption and (B) CO₂ production in WT or B2R^-/- mice fed an HFD for 8 weeks (n = 5 each). (C) Respiratory quotient ratio in WT or B2R^-/- mice fed an HFD for 8 weeks. (n = 5 each). (D) Histochemical analysis of SDH and COX staining in skeletal muscle of 3-month-old WT or B2R^-/- mice (n = 5) Scale bar = 50μm. (E) Dihydroethidium (DHE) and 4’,6-diamidino-2-phenylindole dihydrochloride (DAPI) colocalization. Representative images of gastrocnemius muscle in 3-month-old mouse showing fluorescent labeling of DAPI (blue) in and DHE (red) at the corresponding location and focal plane, with merged pictures, showing colocalization (pink). Scale bar = 20μm. (*P < 0.05; **P<0.005) Data are presented as mean ± S.E.M.