Measurement of Local Partial Pressure of Oxygen in the Brain Tissue under Normoxia and Epilepsy with Phosphorescence Lifetime Microscopy

Cong Zhang; Samuel Bélanger; Philippe Pouliot; Frédéric Lesage

doi:10.1371/journal.pone.0135536

Fig 6

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Obtained pO₂ values in tissue near the focus and surround.

(a) Grayscale angiogram of cortical surface and locations for pO₂ measurement (red: focus; blue: surround). The artery was shown by the red arrows. Scale bar size: 0.2mm (b) Epileptic activity induced a transient dip in tissue pO₂ followed by an increase in pO₂ in the focus. A sustained increase in pO₂ was seen in the surround. The dashed vertical lines show the ictal onset (left) and offset (right). (c) Distribution of percent of initial dip at multiple locations (color dotted) during epileptic activity. The 4-AP injection site is shown by green circle. The artery was shown by the red arrows. Scale bar size: 0.2mm.