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Published online 2015 Jul 27. doi: 10.1073/pnas.1513047112
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Fig. S5.

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Generation of cardiac specific ablation of IP3R2. (A) Schematic illustration of the generation of the IP3R2 floxed mouse (Top) and representative PCR for the genotype detection of WT, IP3R2fl/fl, and IP3R2KO mice. (B) Determination of IP3R2 mRNA levels by real time quantitative reverse transcription PCR (RT-qPCR) analysis of total RNA from isolated cardiomyocytes, using actin as internal standard; each bar represents mean ± SEM of four independent experiments, in each of which reactions were performed in triplicate using the pooled total RNAs from five mice per group. (C) IP3R2 from isolated ventricular cardiomyocytes was immunoprecipitated and immunoblotted. 2,4 DNPH, 2,4-dinitrophenylhydrazone. (D and E) Quantification of data shown in C. Data shown represent means ± SEM from triplicate experiments. *P < 0.05 vs. IP3R2fl/fl, two-tailed t test. (F and G) Levels of IP3R isoforms after cardiac-specific ablation of IP3R2. Determination of IP3R1 (F) and IP3R3 (G) mRNA levels by RT-qPCR analysis of total RNA from isolated cardiomyocytes, using actin as internal standard; each bar represents mean ± SEM of four independent experiments, in each of which reactions were performed in triplicate using the pooled total RNAs from five mice/group.

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