Figure 7

(A) Twice daily i.p. injections of TUDCA for 2 days prior to euthanasia reduced β cell ER stress marker BiP in Akita and db/db-BLKS ER load models. Images are representative of n = 3 biological replicates. (B–G) Two days of twice daily i.p. TUDCA reduced β cell proliferation in 2-week-old Akita mice (B and C; n = 3–5), in 4-week-old db/db mice (D and E; n = 5–13), and in HFD-fed mice (F and G; n = 5–8). Images were acquired at ×200 magnification. Arrowheads point to BrdU-positive β cells. Data are represented as mean ± SEM; *P < 0.05 and **P < 0.01 by ANOVA.