Fig. 4.

Musclin supports physical performance. (A) Schematic of the modified Ostn gene indicating excision of the ATG-containing exon 2 to create the Ostn-KO mouse model. (B) Representative Western blot of musclin from gastrocnemius of WT and Ostn-KO mice. (C) Schematic of the treadmill exercise protocol. Vertical lines indicate mean time points of exercise dropout. (D–F) Summary statistics for treadmill exercise tolerance in terms of duration (D), distance (E), and tolerated workload: E_k + E_p (F). *P < 0.05 Ostn-KO vs. WT. (G) Schematic of a running wheel. (H–J) Summary statistics for voluntary running-wheel exercise performance at day and night in terms of mean velocity (H), duration (I), and distance (J). *P < 0.05 Ostn-KO vs. WT. (K) Schematic of a running wheel and an osmotic pump loaded with musclin (m) or saline (s). (L–N) Summary statistics for voluntary running wheel performance at night in terms of mean velocity (L), duration (M), and distance (N). *P < 0.05 vs. WT saline. KO, Ostn-KO.