Figure 3.

Representative PCR analysis of polymorphic STR for detection of chimerism. DNA was extracted from flow cytometry-purified subcutaneous adipocytes and adipose stromal cells 43 mo after transplant. Figure shows electropherograms for locus D18S51. The figures show the position of a PCR product common to both donor and recipient, as well as donor-specific and recipient-specific products. Analysis of donor-specific and recipient-specific peak areas indicated that ∼12% of the purified adipocyte population (A) and 25% of the stromal cells (B) were derived from donor hematopoietic cells.