Figure 5.

Ploidy analysis demonstrates the absence of cell fusion in the human adipocyte populations. Free-floating adipocytes from 6 subjects were permeabilized and stained with propidium iodide. Figure shows flow cytometric cell cycle analysis with singlet discrimination. ModFit software distinguished peaks for G1 and G2 cells, and events to the right of the G2 peak were considered polyploid. Avg., average.