GloverAdrian GWiklundHelenaRaboneMurielAmonDiva JSmithCraig RO'HaraTimMahChristopher LDahlgrenThomas GAbyssal fauna of the UK-1 polymetallic nodule exploration claim, Clarion-Clipperton Zone, central Pacific Ocean: EchinodermataBiodivers Data J25120162016410.3897/BDJ.4.e7251Benthodytes sanguinolentaThéel, 1882Materials

Type status: Other material. Occurrence: catalogNumber: d0062182-89dc-4deb-b746-688289783b5f; recordNumber: NHM_216; recordedBy: Adrian Glover, Helena Wiklund, Thomas Dahlgren, Maggie Georgieva; individualCount: 1; preparations: tissue and DNA voucher stored in 80% non-denatured ethanol aqueous solution; otherCatalogNumbers: 5023498; associatedSequences: http://www.ncbi.nlm.nih.gov/nuccore/KU519546 | KU519513; Taxon: taxonConceptID: Benthodytes cf. sanguinolenta; scientificName: Benthodytes sanguinolenta; kingdom: Animalia; phylum: Echinodermata; class: Holothuroidea; order: Elasipodida; family: Psychropotidae; genus: Benthodytes; scientificNameAuthorship: Théel, 1882; Location: waterBody: Pacific; stateProvince: Clarion Clipperton Zone; locality: UK Seabed Resources Ltd exploration claim UK-1; verbatimLocality: UK-1 Stratum A; maximumDepthInMeters: 4063; locationRemarks: RV Melville Cruise MV1313; decimalLatitude: 13.9629; decimalLongitude: -116.5513; geodeticDatum: WGS84; Identification: identifiedBy: Diva Amon, David Pawson, Adrian Glover, Helena Wiklund, Thomas Dahlgren; dateIdentified: 2015-06-01; identificationRemarks: identified by DNA and morphology; identificationQualifier: cf; Event: samplingProtocol: Remotely Operated Vehicle; eventDate: 2013-10-15; eventTime: 23:15; habitat: Abyssal plain; fieldNumber: RV03; Record Level: language: en; institutionCode: NHMUK; collectionCode: ZOO; datasetName: ABYSSLINE; basisOfRecord: PreservedSpecimen

Description

Morphologically agrees with either Benthodytes sanguinolenta or Benthodytes typica both from Théel 1882 (Fig. 17).

Genetic data for this taxa with new GenBank accession numbers are provided in Table 2

Diagnosis

Forms a unique monophyletic clade distinct from other AB01 specimens and no match (16S) to any GenBank or BOLD databases. Morphologically consistent with Benthodytes sanguinolenta or B. typica. The type locality of B. sanguinolenta is in the Pacific ocean (34°7'S; 73°56'W, 4000m depth) while type locality of B. typica is Atlantic (35°47'N; 8°23'W, 2000m depth) (Théel 1882). We assign the tentative name Benthodytes cf. sanguinolent to this material until we have a better understanding of genetic variation within the species B. sanguinolenta and B. typica including data from the type localities.

Ecology

Observed moving on the seabed amongst polymetallic nodules.

Benthodytes cf. sanguinolenta Théel, 1882. Specimen NHM_216. (a) Specimen NHM_216 in situ on seafloor shortly before collection by ROV manipulator arm, (b) Live specimen photographed immediately after recovery from the ROV biobox, upper (dorsal view), lower (ventral view). Scale bar 5cm. Image attribution (a) Smith & Amon 2013, (b) Glover, Dahlgren & Wiklund, 2015.

Taxon treatments presented in this paper. Includes Natural History Museum global unique identifier (GUID) which link to the record in the Museum Data Portal (data.nhm.ac.uk), new NCBI GenBank accession numbers (GenBank#), ABYSSLINE Record number (ABYSSLINE_Record#) and NHM Molecular Collection Facility sample ID number (NHMUK_MCF#). Record numbers include both material archived at NHM (all tissue samples and DNA samples, plus some morphological material including whole specimens for some taxa) and material currently housed at the Craig R Smith lab, University of Hawaii (larger 'megafaunal' material indicated by CS numbers in the ABYSSLINE record#). GenBank numbers for data downloaded from GenBank for phylogenetic analysis are presented in Suppl. material 1.

Class Morphological identification GUID ABYSSLINE_ record# NHMUK_ MCF# Genbank_ CO1# Genbank_ 16S# Genbank_ 18S#
Asteroidea Asteroidea sp. (NHM_054) de4bd6ce-07fe-496e-bffc-67a4c6b9782c NHM_054185546311--- KU519512 KU519530
Asteroidea Asteroidea sp. (NHM_054) bc03fc1a-3613-41a2-b1f1-bf905e0fa6d0 NHM_375185546346--- KU519528 ---
Asteroidea Freyastera cf. benthophila b7ffe7a2-7be1-4d4f-b784-7aaecf0ee743 NHM_413 AB01_CS10185546349 KU519550 KU519518 KU519535
Asteroidea Freyastera cf. benthophila 16599946-2aba-4710-98e6-43c522061878 NHM_421185546363 KU519551 ------
Asteroidea Porcellanaster cf. ceruleus c57f1bd3-1b32-41e6-8e1d-0ad6472e4327 NHM_168185546321 KU519568 ------
Asteroidea Porcellanaster cf. ceruleus 7e8ca2d8-aea1-45bd-b7e0-d0575cadd82d NHM_200185546325 KU519569 ------
Asteroidea Porcellanaster cf. ceruleus 95d0bd7f-0df9-47e4-8003-cd12007d54b4 NHM_253185546332 KU519570 KU519525 KU519542
Asteroidea Porcellanaster cf. ceruleus d15a68e0-b2b3-40b4-8cab-0563609cc80d NHM_267185546329 KU519571 ------
Asteroidea Porcellanaster cf. ceruleus 76acc5a2-6e0e-4599-8104-b8e243af10c4 NHM_408185546348 KU519572 ------
Asteroidea Styracaster paucispinus 4ae2430e-549e-47f2-ba5d-0e9a08443d31 NHM_374185546345 KU519573 KU519527 KU519543
Crinoidea Crinoidea sp. (NHM_008) b2a871bf-46d5-4639-a839-427a3efa848c NHM_008185546315 KU519547 KU519514 KU519531
Crinoidea Crinoidea sp. (NHM_055) 280c758b-5287-4a13-9f45-f6a6150b37d0 NHM_055185546310 KU519548 KU519515 KU519532
Crinoidea Crinoidea sp. (NHM_056) 92825c07-a16d-4c5e-a8e9-4fbcdc8cf44a NHM_056185546309--- KU519516 KU519533
Crinoidea Crinoidea sp. (NHM_300) 2866f91e-b99e-4703-a9d3-fe1876df1da1 NHM_300185546328 KU519549 KU519517 KU519534
Holothuroidea Benthodytes cf. sanguinolenta d0062182-89dc-4deb-b746-688289783b5f NHM_216 AB01_CS03185546339 KU519546 KU519513 ---
Holothuroidea Psychropotes cf. semperiana 38c16bec-7bf9-4c2b-b862-5da460ba6c0c NHM_220 AB01_CS05185546335--- KU519526 ---
Ophiuroidea Amphioplus cf. daleus 72db478a-ea4f-4f3e-be08-95ec9fb4d20e NHM_094185546316 KU519544 ------
Ophiuroidea Amphioplus cf. daleus 15e6ddc7-3ca7-453c-bba5-f84888716505 NHM_447 AB01_CS15185546357 KU519545 KU519511 KU519529
Ophiuroidea Ophiomusium cf. glabrum c1c4d8f3-6cd5-439f-a546-943b5e2e8d8f NHM_009185546314 KU519552 ------
Ophiuroidea Ophiomusium cf. glabrum 4d6f6aaf-93fd-4629-b224-2ce8dd3320f6 NHM_124 AB01_CS02185546319 KU519553 ------
Ophiuroidea Ophiomusium cf. glabrum 2ed865af-1605-4d78-8fd8-9c7659781854 NHM_256185546331 KU519554 ------
Ophiuroidea Ophiomusium cf. glabrum 11948cb9-654f-4519-a654-f134380093ea NHM_329 AB01_CS06185546341 KU519555 KU519519 KU519536
Ophiuroidea Ophiomusium cf. glabrum 292bd655-83d6-440f-9668-82dfa4185b04 NHM_335185546342 KU519556 ------
Ophiuroidea Ophiomusium cf. glabrum 68072fc9-3e84-4202-8e97-6c9c0c5fc83d NHM_415 AB01_CS12185546351 KU519557 ------
Ophiuroidea Ophiomusium cf. glabrum 5ad996fe-134a-4625-a404-9d0cdae435d4 NHM_452185546352 KU519558 ------
Ophiuroidea Ophiotholia sp. (NHM_076) bd6fe2ce-b4ae-470e-8bdc-cf28a94c6208 NHM_076185546306 KU519559 KU519520 KU519537
Ophiuroidea Ophiotholia sp. (NHM_076) 97d40306-fe6c-4911-8e68-1f9efc3d838f NHM_078185546305 KU519560 ------
Ophiuroidea Ophiotholia sp. (NHM_076) 479218ae-813b-4736-b3f2-7eec63640ffd NHM_104185546317 KU519561 ------
Ophiuroidea Ophiotholia sp. (NHM_076) 90e22ace-ef5d-4cb5-a4a5-29fcd55ed660 NHM_119185546318 KU519562 ------
Ophiuroidea Ophiuroidea incertae sedis sp. (NHM_041) 608349ff-5adf-4e1e-8cd7-7e0e41aee222 NHM_041185546312 KU519563 KU519521 KU519538
Ophiuroidea Ophiuroidea incertae sedis sp. (NHM_072) 241d094a-568f-4194-997c-fd08f67dcdac NHM_072185546307 KU519564 KU519522 KU519539
Ophiuroidea Ophiuroidea incertae sedis sp. (NHM_303) e9f38ce3-5ed5-49f3-8713-c26de2eefd2b NHM_303185546340 KU519565 KU519523 KU519540
Ophiuroidea Ophiuroidea incertae sedis sp. (NHM_303) 93b0a70d-c74e-4735-b70e-0c6e4c6a36ff NHM_371185546344 KU519566 ------
Ophiuroidea Perlophiura profundissima f263bc90-6307-462c-9e02-7b87d20e2840 NHM_257185546330 KU519567 KU519524 KU519541

Taxa table

Data type: Genbank accession numbers

Brief description: List of taxa downloaded from GenBank that are included in the phylogenetic analyses, with their Genbank accession numbers. Accession numbers for taxa sequenced in this study are found in Table 2.

File: oo_73309.xlsx

Glover et al.ThéelH1882Report on the Holothuroidea dredged by H.M.S. 'Challenger' during the years 1873-764176pp -