Table 2
Measurement of CVI988 vaccine and RB-1B challenge virus in PBL of experimental chickens, using pp38 SNP q-PCR assay.
| Group | Bird # | Virus | Sampling time-point: PBL (virus level: genome copies per 10,000 cells) | Survived S/Died D (time of death dpc) | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 3 dpv | 6 dpv | 10 dpv | 13 dpv | 17 dpv | 24 dpv | 27 dpv | 33 dpv | 40 dpv | 45 dpv | ||||
| −4 dpc | −1 dpc | 3 dpc | 6 dpc | 10 dpc | 17 dpc | 20 dpc | 26 dpc | 33 dpc | 38 dpc | ||||
| A Vacc only (level of CVI) a | 2428 | CVI | —c | 66 | 81 | 43 | 101 | 85 | 79 | 135 | 144 | NDd | S |
| 2433 | CVI | – | 7 | 36 | 31 | 40 | 21 | 15 | 23 | 22 | 18 | S | |
| 2434 | CVI | – | 74 | 75 | 53 | 75 | 57 | 56 | 42 | 10 | 31 | S | |
| 2437 | CVI | 12 | 84 | 110 | 78 | 69 | 93 | 105 | 115 | 137 | 134 | S | |
| 2442 | CVI | – | 12 | 77 | 48 | 45 | 54 | 42 | 38 | 54 | 47 | S | |
| 2444 | CVI | – | 23 | 61 | 29 | 29 | 44 | 31 | 37 | 40 | 35 | S | |
| 2446 | CVI | – | 42 | 9 | ND | 24 | 34 | 15 | 16 | 15 | 9 | S | |
| 2447 | CVI | – | 17 | 192 | 52 | 151 | 129 | 91 | 89 | 92 | 113 | S | |
| B Chall only (level of RB-1B) b | 2401 | RB1B | – | – | 3.1 | 913 | 13231 | 15924 | 30017 | De | D | D | D 20 dpc |
| 2403 | RB1B | – | – | – | 133 | 3138 | D | D | D | D | D | D 11 dpc | |
| 2405 | RB1B | – | – | – | 249 | D | D | D | D | D | D | D 8 dpc | |
| 2421 | RB1B | – | – | – | 336 | 6586 | 2968 | 3783 | 7878 | 16290 | 32241 | S | |
| 2424 | RB1B | – | – | – | 309 | D | D | D | D | D | D | D 10 dpc | |
| 2429 | RB1B | – | – | 57 | 1421 | D | D | D | D | D | D | D 8 dpc | |
| 2432 | RB1B | – | – | – | – | – | – | 4 | 17 | 1270 | – | S | |
| 2435 | RB1B | – | – | – | 35 | 1528 | 1739 | 2632 | 3491 | 9352 | D | D 33 dpc | |
| 2436 | RB1B | – | – | – | 73 | 2171 | 2881 | 5375 | 5391 | 4592 | 4902 | S | |
| 2439 | RB1B | – | 1.1 | 2.8 | 190 | 3631 | 4170 | 8707 | D | D | D | D 24dpc | |
| 2440 | RB1B | – | – | – | 99 | 4400 | 2532 | 3893 | 6842 | 7983 | 14220 | S | |
| 2443 | RB1B | – | – | 4.0 | 225 | 2493 | 8329 | 16123 | D | D | D | D 21 dpc | |
| 2445 | RB1B | – | – | 23 | 697 | D | D | D | D | D | D | D 8 dpc | |
| 2448 | RB1B | – | – | – | 419 | D | D | D | D | D | D | D 8 dpc | |
| C Vacc & Chall (level of CVI and level of RB-1B) | 2404 | CVI | – | 10 | 98 | 31 | 18 | 9 | 12 | 12 | 9 | 7 | S |
| RB1B | – | – | – | – | – | – | – | – | – | 87 | |||
| 2407 | CVI | – | 12 | 44 | 22 | 7 | 12 | 11 | 10 | 11 | 21 | S | |
| RB1B | – | – | – | – | – | – | – | – | – | – | |||
| 2414 | CVI | – | 12 | 53 | 26 | 34 | 32 | 28 | 41 | 50 | 108 | S | |
| RB1B | – | – | – | – | – | – | – | – | – | – | |||
| 2417 | CVI | – | 6 | 25 | 4 | 5 | 2 | 2 | 0.3 | 0.3 | 1 | S | |
| RB1B | – | – | – | – | – | – | – | – | – | – | |||
| 2418 | CVI | – | 3 | 28 | 33 | 24 | 29 | 25 | 21 | 15 | 24 | S | |
| RB1B | – | – | – | – | – | – | – | – | – | – | |||
| 2419 | CVI | – | 6 | 7 | 10 | 11 | 8 | 16 | 9 | 7 | 12 | S | |
| RB1B | – | – | – | – | – | – | – | – | – | – | |||
| 2420 | CVI | – | – | – | – | D | D | D | D | D | D | D 10 dpc | |
| RB1B | – | 0.9 | 0.9 | 460 | D | D | D | D | D | D | |||
| 2422 | CVI | – | 23 | 37 | 23 | 10 | 10 | 11 | 9 | 4 | 2 | S | |
| RB1B | – | – | – | – | – | 2 | 2 | 10 | 50 | 134 | |||
| 2423 | CVI | – | 6 | 20 | 31 | 17 | 10 | 12 | 7 | 4 | 0.3 | S | |
| RB1B | – | – | – | – | – | – | – | – | – | 171 | |||
| 2425 | CVI | – | 9 | 51 | 65 | 22 | 25 | 8 | 4 | 3 | 4 | S | |
| RB1B | – | – | – | – | – | – | – | – | – | 3 | |||
aAll Group A birds were negative for RB-1B at each time-point (data not shown).
bAll Group B birds were negative for CVI988 at each time-point (data not shown).
c—indicates samples that were considered negative in the q-PCR (40-Ct value ≤1) and resulted in a baseline value of 0.6 genome copies per 104 cells.
dND = Not done (chicken inadvertently not sampled at this time-point).
eD = bird dead, therefore no sample at this time-point.