FIG 5

Effect of pH on the pressure inactivation of the HuNoV GII.4-5M strain. HuNoV stock was inoculated into MEM with pH 4.0, 7.0, and 10.0 and treated at 200 MPa for 5 min at 4°C. The surviving viruses were evaluated by a PGM-MB binding assay and real-time RT-PCR. Asterisks denote that the groups are significantly different (P < 0.05). The dashed line indicates the detection limit (2 log₁₀ RNA copies/ml).