FIG 4

Leukocytes appear in the brains and spinal cords of treated mice after DON treatment is stopped. (A) Representative H&E-stained brain (top) and spinal cord (bottom) sections from day 7 (treated and untreated) and day 13 (after DON treatment was stopped at day 7). (B) H&E-stained sections were scored as follows: 0 for no inflammation, 1 for one or two occasional inflammatory foci, 2 for moderate foci in <50% of 10× fields, and 3 for moderate to severe foci in >50% of 10× fields. A score of +1 was given for abundant cellularity. Mice were treated with PBS or 0.3 mg/kg of DON once daily through day 7. Gray shading under the x axis identifies the drug treatment period. Bar = 100 μm. Error bars represent standard errors of the means of data for three mice per time point per group. **, P < 0.01 (as determined by one-way ANOVA). NA, not applicable.