FIG 5

Interactions of hPIV-2 P or V proteins with Graf1 and its mutants. (A) Schematic diagram of full-length (FL) Graf1 and its deletion mutants with the N-terminal myc tag. Missing regions are indicated by the dotted lines. (B and C) COS cells were transfected with the indicated plasmids as described in the legend of Fig. 4B. At 48 hpt, the cell extracts were analyzed directly by IB analysis (anti-V/P and monoclonal anti-myc, as indicated). Immunoprecipitates with monoclonal anti-myc were probed by monoclonal anti-myc or anti-V/P, as indicated. myc-GFP was used to check the nonspecific interaction between the myc tag and V protein. Double and single asterisks indicate immunoglobulin heavy chain and light chain, respectively. All experiments were performed at least three times independently.