Carcinoembryonic Antigen-Related Cell Adhesion Molecule 5 Is an Important Surface Attachment Factor That Facilitates Entry of Middle East Respiratory Syndrome Coronavirus

Che-Man Chan; Hin Chu; Yixin Wang; Bosco Ho-Yin Wong; Xiaoyu Zhao; Jie Zhou; Dong Yang; Sze Pui Leung; Jasper Fuk-Woo Chan; Man-Lung Yeung; Jinghua Yan; Guangwen Lu; George Fu Gao; Kwok-Yung Yuen

doi:10.1128/JVI.01133-16

FIG 1

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CEACAM5 is a cell surface binding protein of MERS-CoV. (A) A549 and NIH 3T3 biotinylated cell membrane proteins were extracted, separated by 4 to 12% gradient gel, and transferred to PVDF membranes. WB, Western blotting. (B) Membrane proteins were probed with MERS-S-pseudotyped virus followed by detection of virus binding by incubation with immune serum directed against the MERS-CoV spike protein (left lane). Nonsusceptible cell membrane extracts from NIH 3T3 was included as a negative control (right lane). (C) The gel fractions corresponding to the positive signal at approximately 90 kDa were cut, electroeluted, and confirmed by VOPBA prior to submission for MS protein identification. (B and C) The position of CEACAM5 is indicated with asterisks. (D) Identified amino acid sequences and their corresponding positions.