FIG 9

CEACAM5 overexpression does not facilitate SARS-CoV entry in VeroE6 cells. (A) VeroE6 cells were transfected with a CEACAM5-expressing plasmid or an empty vector. (B) The VeroE6 cells were challenged with SARS-S pseudovirus or VSV-G pseudovirus at a ratio of 100 LP per cell for 1 h. Luciferase activity from CEACAM5-transfected VeroE6 cells was determined at 48 h after pseudovirus challenge and was normalized to that of the empty-vector-transfected VeroE6 cells. (B) Means and standard deviations were derived from the results of three independent experiments. Statistical analyses were carried out using Student's t test. Statistical significance is indicated by the asterisks (P < 0.05).