Identification of Residues That Affect Oligomerization and/or Enzymatic Activity of Influenza Virus H5N1 Neuraminidase Proteins

Meiling Dai; Hongbo Guo; Jos C. F. M. Dortmans; Jojanneke Dekkers; Johan Nordholm; Robert Daniels; Frank J. M. van Kuppeveld; Erik de Vries; Cornelis A. M. de Haan

doi:10.1128/JVI.01346-16

FIG 1

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Expression of recombinant NAs. (A) Schematic representation of the recombinant full-length NA (FL-NA) and soluble NA proteins. The FL-NA protein contains the NA head, stalk, transmembrane domain (TMD), and cytoplasmic tail (CT). The soluble NA protein contains the head and stalk regions fused with a tetramerization domain (TD) and a cleavable signal sequence (SS). (B) Recombinant full-length NA (FL-NA) and soluble GCN4-containing NA (GCN4-NA) proteins HN, VN, TK, and HB were expressed in HEK293T cells, and their expression levels were analyzed by SDS-PAGE followed by Western blotting as described in Materials and Methods. The position on the gel of the relevant molecular mass markers is shown.